摘要:为了鉴定家驴ETS基因家族成员的组成并分析其特征,研究运用生物信息学方法从进化关系、染色体定位、基因结构、蛋白保守基序、组织表达和功能富集等方面进行了分析。结果表明:通过BLAST比对,在家驴基因组中鉴定到26个ETS基因家族成员,氨基酸数量为213~662个,相对分子质量为24 879.08～70 700.89 Da,等电点为4.88～9.32。26个ETS家族成员在13条染色体上随机不均匀分布,二级结构主要以无规则卷曲为主,其次是α-螺旋,β-转角含量最少。亚细胞定位结果显示,大部分ETS家族成员位于细胞核。根据系统发育分析,驴ETS基因家族成员可分为12个亚家族,各个亚家族成员分别聚集,在基因结构和蛋白理化性质上具有相似性。保守基序分析发现,ETS蛋白中存在10种保守基序,其中motif 1、motif 2、motif 3在所有ETS成员中均存在,三者共同组成ETS结构域。8个ETS蛋白中含有motif 5和motif 6,两者构成了PNT结构域。转录组数据显示26个ETS家族成员在13种不同组织中表达水平存在差异,其中ETS1在血液中高表达,ELF3在盲肠中高表达,SPI1在心脏中高表达。本研究结论不仅对驴ETS基因家族进行了系统分析,同时为深入探究该家族生物学功能以及了解驴的生理特性提供了线索。
Abstract:To identify the composition and analyze the characteristics of the ETS gene family members in domestic donkeys,this study used bioinformatics methods to analyze evolutionary relationships,chromosomal localization,gene structure,protein conserved motifs,tissue expression,and functional enrichment.The results showed that 26 ETS gene family members were identified in the donkey genome through BLAST alignment,with amino acid numbers ranging from 213 to 662,relative molecular weights ranging from 24 879.08 to 70 700.89 Da,and isoelectric points ranging from 4.88 to 9.32.26 ETS family members are randomly and unevenly distributed on 13 chromosomes,with the secondary structure mainly consisting of irregular coils,followed by alpha helices,and the least content of beta turns.The subcellular localization results showed that most members of the ETS family are located in the nucleus.According to phylogenetic analysis,the ETS gene family members of donkeys can be divided into 12 subfamilies,and each subfamily member is clustered separately,with similarities in gene structure and protein physicochemical properties.Conservative motif analysis revealed the presence of 10 conserved motifs in ETS proteins,among which motif 1,motif 2,and motif 3 are present in all ETS members and together form the ETS domain.The 8 ETS proteins contain motif 5 and motif 6,which form the PNT domain.Transcriptome data showed differential expression levels of 26 ETS family members in 13 different tissues,with ETS1 highly expressed in the blood,ELF3 highly expressed in the cecum,and SPI1 highly expressed in the heart.The conclusion of this study not only provides a systematic analysis of the ETS gene family in donkeys,but also provides clues for further exploring the biological functions of this family and understanding the physiological characteristics of donkeys.